Animal Sera

Adult Bovine Serum - Sterile Filtered (ABSF)


Adult Bovine Serum - Sterile Filtered (ABSF)

Adult Bovine Serum (ABSF) is obtained from blood taken from cattle deemed fit for human consumption following ante and post mortem veterinary inspection.  The blood is collected in slaughter-houses supervised by government veterinarians. 

ABSF is prepared form Adult Bovine Serum - Raw (ABSR).

STERILE FILTERED: Semi-processed Raw Adult Bovine Serum, is thawed, pooled and filtered through a series of sterile membranes down to 0.2 microns before being finally packaged, usually in 500ml or 1L bottles. No further processing, additions or deletions are performed.

Available Treatments - Adult Bovine Serum - Sterile Filtered (ABSF)
IRRADIATED Gamma Irradiation of Sterile Filtered ABSF takes place in Brisbane at an Australian Government licensed gamma irradiation facility. Frozen serum is irradiated following Moregate Biotech's validated procedure to a customer specified dose range. A gamma irradiation indicator (colour change) is applied to every container before irradiation. A Certificate of Irradiation is issued for every lot of irradiated serum.
SPECIFICATION - Adult Bovine Serum - Sterile Filtered (ABSF)
Product Adult Bovine Serum - Sterile Filtered
Catalogue No. ABSF
Source Bovine blood from healthy cattle which have been inspected ante and post mortem by Australian or New Zealand Government veterinarians and passed as fit for human consumption.
Collection Method Serum (ABSR) is passed through a series of filters terminating with 0.2 micron pore size sterilising grade filters. Product is dispensed in ISO 14644 Class 5 Laminar Flow Workstation via a closed system. It is then immediately frozen.
Description A clear amber viscous liquid with a characteristic odour.
Pack Size 500ml & 1000ml sterile PET & PETG square media bottles
Storage -20 degrees Centigrade
Expiry Date Six years from the date of manufacture
Test Method Specification
Visual Examination Visual Satisfactory
Viruses Modified 9CFR (113 - 53c)
Bovine Viral Diarrhea Virus Fluorescent Antibody Not detected
Infectious Bovine Rhinotracheitis Cytopathic Agents Not detected
Para Influenza 3 (PI3) Haemadsorbing Agents Not detected
Sterility Millipore Steritest Broth Culture 14 Day Incubation Sterile
Mycoplasma Mycoplasma Broth Culture 35 Day Incubation Not detected
pH pH meter As reported
Osmolality Osmometer 240-320 mOsmol/kg
Total Protein Beckman Coulter Synchron Clinical Systems 45 - 85 mg/ml
Albumin Electrophoresis As reported
Haemoglobin Haemoglobin UV/VIS Spectrophotometer < 4 mg/ml
Endotoxin Kinetic Turbidimetric ≤ 50 IU/mL
Cell Line Used
Hybridoma: Cell Culture ≥ 85% of FCS Control
MRC-5: Cell Culture ≥ 85% of FCS Control

The European Pharmacopeia consists of a number of general and specific monographs covering various classes of products.

The monographs set out requirements to be met and followed for all products in the class.

Recently the EP has introduced a Monograph for Bovine Serum – Monograph No 04/2006:2262 to be found in E.P. 5.4

This monograph provides a definition of Bovine Serum and sets out details of production, and requirements for viral inactivation parameters, quality control testing, storage conditions and labelling.

Moregate Bovine Serum products meet these requirements and Certificates of Analysis are modeled on the QC test requirements.


The United States Pharmacopeia-National Formulary (USP-NF) contains standards for medicines, dosage forms, drug substances, excipients, medical devices, and dietary supplements.

Within the Pharmacopeia are Monographs and general chapters.

The monographs consist of information such as the ingredient name, definition, packaging and labelling requirements, storage and a specification.

The specifications list tests to be performed along with the procedure to be followed and the acceptable limit.

The European Medicines Agency (EMEA) is a decentralised body of the European Union with headquarters in London. It sits alongside the European Directorate for the Quality of Medicines (EDQM).

The main responsibility is the protection and promotion of public and animal health, through the evaluation and supervision of medicines for human and veterinary use.

The EMEA publishes guidelines on quality, safety and efficacy testing requirements.

These guidelines are prepared by committees, and those guidelines which relate to the use of Bovine Serum, including Fetal Bovine Serum, in the manufacture of medicines include:

CPMP: Committee for Proprietary Medicinal Products

Note for Guidance on the Use of Bovine Serum in the Manufacture of Human Biological Medicinal Products

Note for Guidance on Minimising the Risk of Transmitting Animal Spongiform Encephalopathy Agents via Human and Veterinary Medicinal Products. (Also adopted by the CVMP)

CVMP: Committee for Veterinary Medicinal Products

Requirements and Controls applied to Bovine Serum used in the production of Immunological Veterinary Medicinal products.

These Guidelines include requirements for Virus Testing of Fetal Bovine Serum and other Bovine Serum. Further reference to these requirements can be found in “Virus Testing”. 

All Moregate Biotech Fetal Bovine Serum and other Bovine Serum meet the requirements of the Guidelines in relation to Fetal Bovine Serum and Bovine Serum.

Ingredients of animal origin used in the United States of America, in the manufacture of veterinary and human biologics are required to be in compliance with the Code of Federal Regulations, Title 9 – Animals and Animal Products, Chapter 1 - Animal and Plant Health Inspection Service, Department of Agriculture Part 113-53c, commonly referred to as 9CFR Part 113-53c.

This legislation sets out the requirements for detection of extraneous viruses, detailing the methods to be used and the list of viruses that shall be tested for.  

Over time 9CFR Part 113-53c became the accepted standard for the testing of animal sera, particularly Fetal Bovine Serum for adventitious viral agents.

Bovine Respiratory Syncytical Virus, Bovine Viral Diarrhea Virus, Bovine Parvovirus, Bluetongue Virus, Bovine Adenovirus, Rabies Virus and Reovirus are tested for by fluorescent antibody

Infections Bovine Rhinotracheitis tested for by Cytopathic Agents.

Infectious Influenza 3 tested for by Haemadsorbing Agents

Hazards Identification

  • Not hazardous
  • Get Medical attention immediately

First Aid Measures
Get Medical attention immediately.

If swallowed, give several glasses of water to drink to dilute.

Skin Contact
Wash skin with soap and copious amounts of water.

Eye Contact
Flush with water for at least 15 minutes, lifting upper and lower eyelids occasionally.

Accidental Release

Procedures for Personal Precaution
Exercise appropriate precautions to minimize direct contact with skin or eyes.

Methods for Cleaning Up
Mop up
Ventilate area and wash spill site after material pickup is complete

Handling and Storage

Normal measures for preventive fire protection

Keep tightly closed under correct storage conditions

Exposure Controls / Personal Protection

  • Wash thoroughly after handling
  • Protective gloves

Disposal Considerations
Contact a licensed professional waste disposal service to dispose of this material.

Transport Information

  • Non-hazardous for road transport
  • Non-hazardous for sea transport
  • Non-hazardous for air transport

Note: The above information is believed to be correct, but shall be used as a guide only.

Disclaimer: For pharmaceutical use only.